Coding
Part:BBa_J45000:Design
Designed by: Bartholomew Canton Group: iGEM06_MIT (2006-06-07)
Smell Enzyme
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 4
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 4
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 4
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 4
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
I hate this sort of restriction
this enzyme has a Km = 83μM
Source
Pichersky??